XpressRNA Cell Kit
XpressRNA Cell Kit provide easy, quick and efficient method for total RNA extraction from fresh human saliva, bacteria and cultured cell samples. The XpressRNA technology provides magnetic bead based method to purify RNA with high quality indices as evident from the A260/280 ratio >1.8, high RIN scores and minimal genomic DNA contamination. Purified RNA can be used for downstream applications including cDNA synthesis, RT-qPCR, Next-Gen Sequencing and expression array assays.
Highlights
- Quick and efficient method for RNA isolation using magnetic beads in less than an hour
- No phenol/chloroform extraction, no LiCl or ethanol precipitation needed
- Deliver high quality and ready-to-use total RNA for cDNA synthesis, RT-qPCR, Next-Gen Sequencing, and microarray
Specifications | Saliva | Bacteria | Cultured Cells |
---|---|---|---|
Format | Magnetic bead | ||
Sample type | Whole saliva | Bacterial culture | Cultured cells |
Starting material | 0.2 ml | OD ≤ 0.8 | Up to 1×10^6 cells |
Elution volume | 30-50 μl | 50-100 μl | 30-50 μl |
Time per prep | 45 minutes | ||
Purification target | Total RNA | ||
Yield* | 3-10 μg | 5.5-35 μg | 0.25-30 μg |
Purity | 260/280 ≥ 2.0 | ||
RIN | ≥8.0 | ||
Storage condition | MagNa mix and Proteinase K at 2-8℃ | ||
Shipping condition | Room Temperature |
*Yield varies due to sample heterogeneity
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