XpressAutomag Blood DNA Kit is designed for optimal automated extraction of high-quality DNA from blood with a starting volume of 200 μl with an approximate yield of ≥ 5 μg of DNA. The kit is well suitable for the instruments Kingfisher Flex, Zybio and Alta, it can process from 1-96 samples in a single run. The Kit provides patented magnetic nanoparticle-based DNA purification from whole blood samples. XpressAutomag Blood DNA Kit is based on a direct blood lysis method that involves minimal handling and effective removal of contaminants and inhibitors. DNA extracted using XpressAutomag Blood DNA Kit is amenable for all downstream applications such as PCR, Real-time PCR, restriction digestion, and sequencing applications. The kit can be easily adapted to automated magnetic- based separation instruments and workstations.
Product highlights
- Isolate high-quality gDNA from fresh/ frozen/ or aged blood samples
- Fast, reproducible, and easy processing using magnetic bead based system
- No centrifugation involved
- Isolate gDNA from blood stored in different vacutainers (EDTA, Heparin, Citrate, and Fluoride)
- Recovered gDNA is compatible with various downstream applications
- Automation friendly
Performance and Data
Extraction of DNA from different blood storage vacutainers
Table 1: Nanodrop data of the gDNA extracted from different blood storage vacutainers
Storage | Conc. (ng/μl) | A260/280 | A260/230 | Yield (μg) |
EDTA | 118.3 | 1.93 | 1.85 | 5.92 |
Citrate | 120.6 | 1.93 | 1.85 | 6.03 |
Heparin | 116.2 | 1.94 | 1.77 | 5.81 |
Fluoride | 127.7 | 1.93 | 1.84 | 6.39 |
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