Plant Molecular Biology has transitioned from basic culture to genetic modifications, crop improvement, transgenic, in-vitro tissue culture etc. The starting step for any of these techniques is DNA extraction. The DNA extraction method used needs to be agile, flexible, economic and fast with minimal steps. Any protocol adopted has to breakdown the rigid cellulose components of the plant cell wall and at the same time extract contaminant free high molecular weight DNA with minimal toxic chemicals usage.
Commonly used methods for Plant DNA extraction
The most commonly used methods for DNA extraction from plants are CTAB and Spin Column extraction method.
CTAB is the most conventional method followed all over. It is added to the plant samples for breakdown of cell wall followed by separating the DNA from rest of the mixture using an extraction buffer. The DNA purified with a variety of organic solvents and alcohols, including hazardous agents like phenol and chloroform.
Spin Column Method
Spin Columns are made up of silica gel based membrane that binds the DNA. During extraction the proteins and organic phase are trapped behind the silica column, while the DNA remains above the layer of gel polymer. The DNA from the aqueous layer is purified and dissolved in TE buffer in a fresh tube.
Comparison of CTAB and Spin Column
|Method||Molecule Of Affinity||Advantage||Disadvantage|
|CTAB Method||DNA, RNA, Protein||Easy EconomicalEfficient for hard to lyse samples||Usage of toxic chemicalsTime consuming protocolFurther purification required before downstream processing|
|Spin Column Method||DNA, RNA, Protein||ReproducibleHigh purity DNA||Not able to recover small DNA fragments Only one time use columnNot suitable for processing bulk samples|
Choosing a right method that fits all our requirements can be quite a tedious task. A hassle free method which is less time consuming that can give high quality, contaminant free and high molecular weight DNA from plant samples is definitely a catch for every researcher. Fortunately there are specific DNA kits available in plenty, but choosing a right kit that checks all the criteria is still a task.
Hence, introducing XpressDNA Plant kit that aims to provide utmost quality at an economical range, without any laborious step with minimal chemical usage.
XpressDNA Plant Kit
Our patented magnetic nanoparticle based technology is used for extraction of pure, contaminant free plant genomic DNA. It is a universal kit for extraction of DNA from different Plants parts such as Leaf, dry seed, germinated seed, seedling, stem and root. This method requires no phase separation and less centrifugation step.
Our optimized protocol is relatively simple to execute by first preparing a Plants sample lysate using our lysis buffer, followed by a DNA binding step using our specially formulated and patented magnetic nanoparticle mix. The bound DNA is immobilised with the help of our MagNA stand made up of six rare strong earth magnets. After a brief washing step, ultra-pure DNA can be easily eluted in a fresh tube at the end of the procedure.
This carefully designed technology and protocol breakdowns cellulose and removes polysaccharides, lipids and various PCR inhibitors providing highly pure DNA, with good molecular weight. Furthermore, our uncoated magnetic nanoparticles, reduces contamination making the extraction products ready for various downstream applications.
Our kits are optimized to be used in batch processes with a multitude of samples and is relatively easy to execute, being one of the best choices for automation, high-throughput applications, and high sample processivity.
Contact us for more information on our kits and check the product page for further details on “XpressDNA Plant Kit”