XpressDNA Plasmid DNA Extraction Kit allows rapid and efficient isolation of plasmid DNA from gram negative bacteria. The extraction and purification of plasmid DNA is carried out using our unique and reliable patented magnetic nanoparticles-based technology. This method allows the isolation of low, medium and high copy number plasmids with high purity and negligible genomic DNA contamination. The complete experiment can be finished within 35-40 minutes. A 1.5 ml overnight culture of gram negative bacteria with OD600 ≦ 1 can give a yield of 2-12ug of plasmid DNA with our kit. The extracted plasmid is suitable for use in downstream applications including PCR, restriction enzyme digestion, cloning, transformation, transfection etc.
XpressDNA plasmid Kit Highlights
- Simple and single step lysis of the bacterial cell for plasmid extraction
- Proprietary DNA binding and washing steps ensuring pure and high DNA yield.
- No contamination of genomic DNA and other proteins in the extracted plasmids
- Gives good results even with low copy number plasmids.
XpressDNA Plasmid Kit Contents
- Plasmid XT Buffer
- Plasmid MagNa mix
- Plasmid Wash Buffer
- Plasmid Elution buffer
- MagNa stand (optional)
Shipping and Storage Conditions
The XpressDNA Plasmid DNA Extraction kit is shipped at 4ºC and on arrival XT buffer and MagNa mix should be stored at 4ºC. All other solutions should be kept tightly sealed and stored at room temperature.
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