XpressAutomag Cell RNA kit is designed for optimal automated extraction of high-quality RNA from various sources including cultured mammalian cells, PBMC, saliva and bacteria. Detectable amount of RNA can be extracted from as low as 20×10³ cultured cells with high purity ratios. It can process 1-96 samples in a single run. The kit is based on patented bare magnetic nanoparticle-based RNA extraction technology. RNA extracted using XpressAutomag Cell RNA kit is amenable to all downstream applications, such as PCR, cDNA synthesis, Real-time PCR and sequencing. The kit can easily be adapted to automated magnetic bead-based workstations (Kingfisher Flex and Kingfisher Apex etc).
Product Highlights:
- Extract high quality RNA from sources including cultured mammalian cells, PBMC, saliva, buccal swab and bacteria
- Fast, reproducible and easy processing using magnetic bead-based system
- No centrifugation involved
- Automation ready
- Extracted RNA compatible with downstream applications
- No residual DNase activity in eluted RNA, if opted for DNase treatment as per the protocol
Performance & Data
Extraction of RNA from :
L
106 cultured mammalian cells
Refer to Protocol A: Extraction from > 105 – 106 cells
L
Gram negative bacteria
Refer to Protocol A: Extraction from > 105 – 106 cells
L
20×103 cultured mammalian cells
Refer to Protocol B: Extraction from 20×103 – 105 cells
| Conc. (ng/µl) | A 260/280 | A 260/230 | Yield (µg) |
| Cultured mammalian cells (106) | |||
| 847.21 | 2.12 | 2.15 | 25.4 |
| 596.64 | 2.09 | 2.11 | 18.0 |
| Cultured mammalian cells (20×10³) | |||
| 20.82 | 1.91 | 1.02 | 0.624 |
| 17.5 | 2.07 | 1.24 | 0.525 |
| Gram Negative Bacteria | |||
| 628.67 | 2.12 | 2.20 | 31.4 |
Extraction of RNA from Human PBMC:
Agilent Tapestation gel profile:
Healthy Donor Patient Sample
Refer to Protocol A: Extraction from >1×105-1×106 cells
| Sample | Con (ng/μL) | A260/280 | Yield (ng) |
| Healthy Donor PBMC | 12.34 | 1.84 | 617 |
| 18.77 | 1.83 | 938.5 | |
| Patient PBMC | 30.44 | 2.12 | 1522 |
| 13.48 | 2.22 | 674 |
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